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Addgene

pDEST47-ARL4D
(Plasmid #67468)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-DEST47
  • Backbone manufacturer
    Life Technologies
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARL4D
  • Alt name
    pRK16s-D47
  • Species
    H. sapiens (human)
  • Mutation
    bp 272 A to C; N91T mutation; bp 327 T to G; silent mutation; bp 390 C to G; silent mutation
  • Entrez Gene
    ARL4D (a.k.a. ARF4L, ARL6)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the insert contains its native stop codon and does not produce a fusion protein with GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST47-ARL4D was a gift from Richard Kahn (Addgene plasmid # 67468 ; http://n2t.net/addgene:67468 ; RRID:Addgene_67468)

  • For your References section:

    Tool box: Plasmids for the expression or knockdown of human ARF Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria with N-myristoyltransferases. Kerr SC, Kahn RA. Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep. 10.1080/21592799.2015.1090523 PubMed 27057421
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