pDEST47-ARF4-GFP
(Plasmid #67392)

• Purpose: Mammalian expression of hArf4 with C-term GFP tag
• Depositing Lab: Richard Kahn
• Publication: Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.

Backbone

• Vector backbone: pcDNA-DEST47
• Backbone manufacturer: Life Technologies
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ARF4
• Alt name: pRK3n-D47
• Species: H. sapiens (human)
• Mutation: V53A, L123P and M134I mutations were unintended PCR-generated artifacts
• Entrez Gene: ARF4 (a.k.a. ARF2)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: GFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid contains the V53A, L123P and M134I mutations in ARF4 from the parent vector pDONR221-ARF4-no stop (Addgene plasmid # 67311). These mutations were unintentionally introduced during PCR.