pDSHA-SAR1-Myc
(Plasmid
#67367)
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PurposeMammalian expression of hSar1 with C-term Myc tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67367 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDS_Myc-X
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSAR1
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Alt namepRK20n-Myc
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SpeciesH. sapiens (human)
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Entrez GeneSAR1A (a.k.a. SAR1, SARA1, Sara, masra2)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDSHA-SAR1-Myc was a gift from Richard Kahn (Addgene plasmid # 67367 ; http://n2t.net/addgene:67367 ; RRID:Addgene_67367) -
For your References section:
Tool box: Plasmids for the expression or knockdown of human ARF Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria with N-myristoyltransferases. Kerr SC, Kahn RA. Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep. 10.1080/21592799.2015.1090523 PubMed 27057421