pDONR221-ARF6
(Plasmid
#67314)
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PurposehArf6 with stop codon in Gateway Entry vector
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67314 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies
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Vector typeBacterial Expression ; Gateway Entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARF6
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Alt namepRK5s-221
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SpeciesH. sapiens (human)
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Entrez GeneARF6
- Promoter None
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-Fwd
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-ARF6 was a gift from Richard Kahn (Addgene plasmid # 67314 ; http://n2t.net/addgene:67314 ; RRID:Addgene_67314) -
For your References section:
Tool box: Plasmids for the expression or knockdown of human ARF Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria with N-myristoyltransferases. Kerr SC, Kahn RA. Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep. 10.1080/21592799.2015.1090523 PubMed 27057421