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Addgene

pDONR221-ARF4-no stop
(Plasmid #67311)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67311 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Life Technologies
  • Vector type
    Bacterial Expression ; Gateway Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARF4
  • Alt name
    pRK3s-221
  • Species
    H. sapiens (human)
  • Mutation
    V53A, L123P and M134I mutations were unintended PCR-generated artifacts
  • Entrez Gene
    ARF4 (a.k.a. ARF2)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that pDSHA-ARF4-Myc and pDEST47-ARF4-GFP (Addgene plasmid #s 67350 & 67392) were created from this plasmid and also contain the V53A, L123P and M134I mutations in ARF4. These mutations were unintentionally introduced during PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-ARF4-no stop was a gift from Richard Kahn (Addgene plasmid # 67311 ; http://n2t.net/addgene:67311 ; RRID:Addgene_67311)

  • For your References section:

    Tool box: Plasmids for the expression or knockdown of human ARF Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria with N-myristoyltransferases. Kerr SC, Kahn RA. Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep. 10.1080/21592799.2015.1090523 PubMed 27057421
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