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Addgene

pET28b(+)-TM0848
(Plasmid #67287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6461
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    To produce protein, transform into Rosetta2(DE3)pLysS cells.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GTP-binding protein YqeH
  • Alt name
    YqeH
  • Species
    Thermotoga maritima
  • Insert Size (bp)
    1092
  • GenBank ID
    Q9WZV2
  • Promoter T7
  • Tag / Fusion Protein
    • N tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b(+)-TM0848 was a gift from SeWon Suh (Addgene plasmid # 67287 ; http://n2t.net/addgene:67287 ; RRID:Addgene_67287)
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