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pMJH65
(Plasmid #67273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMJH46
  • Backbone size w/o insert (bp) 8754
  • Total vector size (bp) 10254
  • Vector type
    Bacterial Expression ; Red Recombinase, temperature sensitive

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    SM10lambdapir
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tetA
  • Alt name
    Tetracycline resistance gene
  • Insert Size (bp)
    1500
  • Promoter tetA promoter of plasmid pACYC184 origin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstZ17I (unknown if destroyed)
  • 3′ cloning site SfiI (unknown if destroyed)
  • 5′ sequencing primer GTAGACTTCCGTTGAACT
  • 3′ sequencing primer TAATGAATCGGCCAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Conjugally transferable red recombinase vector pMJH65

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMJH65 was a gift from Mark Liles (Addgene plasmid # 67273 ; http://n2t.net/addgene:67273 ; RRID:Addgene_67273)

  • For your References section:

    Genome modifications and cloning using a conjugally transferable recombineering system. Hossain MJ, Thurlow CM, Sun D, Nasrin S, Liles MR. Biotechnol Rep (Amst). 2015 Aug 28;8:24-35. doi: 10.1016/j.btre.2015.08.005. eCollection 2015 Dec. 10.1016/j.btre.2015.08.005 PubMed 28352570
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