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Addgene

TbSIR2RP1
(Plasmid #67242)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMalC2XHU
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 7653
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TbSIR2RP1
  • Species
    Trypanosoma brucei
  • Insert Size (bp)
    1053
  • GenBank ID
    AF102869.1
  • Promoter p-lac
  • Tags / Fusion Proteins
    • none
    • MBP, TEV (N terminal on backbone)
    • Thrombin, HIS (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer 5'- TGC GTA CTG CGG TGA TCA AC -3'
  • 3′ sequencing primer 5'- CTG CAA GGC GAT TAA GTT GG -3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TbSIR2RP1 was a gift from Cynthia Wolberger (Addgene plasmid # 67242 ; http://n2t.net/addgene:67242 ; RRID:Addgene_67242)

  • For your References section:

    Side chain specificity of ADP-ribosylation by a sirtuin. Fahie K, Hu P, Swatkoski S, Cotter RJ, Zhang Y, Wolberger C. FEBS J. 2009 Dec;276(23):7159-76. doi: 10.1111/j.1742-4658.2009.07427.x. Epub 2009 Nov 6. 10.1111/j.1742-4658.2009.07427.x PubMed 19895577
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