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PurposeExpresses N-His tagged nonglycosylated human serum transferrin in mammalian cells
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNUT
- Backbone size w/o insert (bp) 7400
- Total vector size (bp) 9500
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Vector typeMammalian Expression
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Selectable markersMethotrexate
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman serum transferrin
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Alt namehTF
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SpeciesH. sapiens (human)
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Insert Size (bp)2136
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MutationAsn413 Asp, Asn611Asp
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GenBank IDAF28814
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Entrez GeneTF (a.k.a. HEL-S-71p, PRO1557, PRO2086, TFQTL1)
- Promoter SV40
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Tag
/ Fusion Protein
- N-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer CCTTTGCGCCCGGACTCG
- 3′ sequencing primer GTGGCAACTTCCAGGGCC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe laboratory of Dr. Ross T.A. MacGillivray at the University of British Columbia in Vancouver BC Canada
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNUT N6His hTFNG was a gift from Ross MacGillivray & Anne Mason (Addgene plasmid # 67240 ; http://n2t.net/addgene:67240 ; RRID:Addgene_67240) -
For your References section:
Differential effect of a his tag at the N- and C-termini: functional studies with recombinant human serum transferrin. Mason AB, He QY, Halbrooks PJ, Everse SJ, Gumerov DR, Kaltashov IA, Smith VC, Hewitt J, MacGillivray RT. Biochemistry. 2002 Jul 30;41(30):9448-54. bi025927l [pii] PubMed 12135367