pCS2 xRor2-EGFP
(Plasmid
#67163)
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Purposemammalian expression / in vitro transcription
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67163 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4085
- Total vector size (bp) 7629
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Vector typeMammalian Expression ; in vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTyrosine-protein kinase transmembrane receptor ROR2
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Alt nameROR2
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SpeciesX. laevis (frog)
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Insert Size (bp)3582
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Entrez Generor2 (a.k.a. xror2)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 xRor2-EGFP was a gift from Alexandra Schambony (Addgene plasmid # 67163 ; http://n2t.net/addgene:67163 ; RRID:Addgene_67163) -
For your References section:
Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA. Feike AC, Rachor K, Gentzel M, Schambony A. Biochem Biophys Res Commun. 2010 Oct 1;400(4):500-6. doi: 10.1016/j.bbrc.2010.08.074. Epub 2010 Aug 21. 10.1016/j.bbrc.2010.08.074 PubMed 20732301