pCS2 xRor2-mCherry
(Plasmid #67162)

• Purpose: mammalian expression / in vitro transcription
• Depositing Lab: Alexandra Schambony
• Publication: Feike et al Biochem Biophys Res Commun. 2010 Oct 1;400(4):500-6. doi: 10.1016/j.bbrc.2010.08.074. Epub 2010 Aug 21.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4085
• Total vector size (bp): 7592
• Vector type: Mammalian Expression ; in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tyrosine-protein kinase transmembrane receptor ROR2
• Alt name: ROR2
• Species: X. laevis (frog)
• Insert Size (bp): 3507
• Entrez Gene: ror2 (a.k.a. xror2)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry