pUMV19
(Plasmid
#67161)
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Purposeinducible operation of the mevalonate pathway in E. coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67161 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC118
- Backbone size w/o insert (bp) 3200
- Total vector size (bp) 9900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namethe mevalonate pathway genes
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Alt nameHMGS, HMGR, IDI, MVK, PMVK, DPMVD
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SpeciesStreptomyces sp. CL190
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Insert Size (bp)6792
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GenBank IDAB037666
- Promoter lacZ
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (destroyed during cloning)
- 3′ cloning site SnaBI (destroyed during cloning)
- 5′ sequencing primer M13 Rev
- 3′ sequencing primer M13 F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Takahashi S et al J Bacteriol 1999 Feb;181(4):1256-63.
Kaneda K et al Proc Natl Acad Sci USA 2001 Jan 30;98(3):932-7.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUMV19 was a gift from Tomohisa Kuzuyama (Addgene plasmid # 67161 ; http://n2t.net/addgene:67161 ; RRID:Addgene_67161) -
For your References section:
A gene cluster for the mevalonate pathway from Streptomyces sp. Strain CL190. Takagi M, Kuzuyama T, Takahashi S, Seto H. J Bacteriol. 2000 Aug;182(15):4153-7. PubMed 10894721
Map uploaded by the depositor.
