Fast-GCaMP6f-RS09
(Plasmid
#67160)
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Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activity
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67160 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGP-RSET
- Backbone size w/o insert (bp) 2770
- Total vector size (bp) 1353
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFast-GCaMP6f-RS09
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SpeciesH. sapiens (human); jellyfish
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Insert Size (bp)1353
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MutationL414T within GCaMP6f
- Promoter T7
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Tags
/ Fusion Proteins
- 6xHis (N terminal on insert)
- T7 tag (N terminal on insert)
- Xpress tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CAT GGT CCT GCT GGA GTT CGT G
- 3′ sequencing primer CGT CGC CGT CCA GCT CGA CCA (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Fast-GCaMP6f-RS09 was a gift from Samuel Wang (Addgene plasmid # 67160 ; http://n2t.net/addgene:67160 ; RRID:Addgene_67160) -
For your References section:
Fast calcium sensor proteins for monitoring neural activity. Badura A, Sun XR, Giovannucci A, Lynch LA, Wang SS. Neurophotonics. 2014 Oct;1(2):025008. 10.1117/1.NPh.1.2.025008 PubMed 25558464