Fast-GCaMP6f-RS06
(Plasmid #67159)

• Purpose: genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
• Depositing Lab: Samuel Wang
• Publication: Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Backbone

• Vector backbone: pGP-RSET
• Backbone size w/o insert (bp): 2770
• Total vector size (bp): 1353
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fast-GCaMP6f-RS06
• Species: H. sapiens (human); jellyfish
• Insert Size (bp): 1353
• Mutation: M374Q within GCaMP6f
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • T7 tag (N terminal on insert)
  • Xpress tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: CAT GGT CCT GCT GGA GTT CGT G
• 3′ sequencing primer: CGT CGC CGT CCA GCT CGA CCA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry