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Addgene

pSG5 N-HA SADBs shRNA resistant
(Plasmid #67157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone size w/o insert (bp) 4076
  • Total vector size (bp) 5217
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Brsk1
  • Alt name
    SadB
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1144
  • Mutation
    Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resistant)
  • Entrez Gene
    Brsk1 (a.k.a. Gm1100, SAD-B, SADB)
  • Promoter SV40 promoter
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5 N-HA SADBs shRNA resistant was a gift from Maria Alvarado-Kristensson & Ana Carrera (Addgene plasmid # 67157 ; http://n2t.net/addgene:67157 ; RRID:Addgene_67157)

  • For your References section:

    SADB phosphorylation of gamma-tubulin regulates centrosome duplication. Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera AC. Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 2009 Aug 2. 10.1038/ncb1921 PubMed 19648910
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