pEGFP C1 SADBs shRNA Resistant
(Plasmid
#67155)
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PurposepEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67155 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 4200
- Total vector size (bp) 5425
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBrsk1
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Alt nameSadB
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SpeciesM. musculus (mouse)
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Insert Size (bp)1032
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Mutationnucleotide 294 C-T, nucleotide 297 C-G
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Entrez GeneBrsk1 (a.k.a. Gm1100, SAD-B, SADB)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP C1 SADBs shRNA Resistant was a gift from Maria Alvarado-Kristensson & Ana Carrera (Addgene plasmid # 67155 ; http://n2t.net/addgene:67155 ; RRID:Addgene_67155) -
For your References section:
SADB phosphorylation of gamma-tubulin regulates centrosome duplication. Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera AC. Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 2009 Aug 2. 10.1038/ncb1921 PubMed 19648910