Beta-2-adrenergic receptor-mRFP
(Plasmid #67145)

• Purpose: Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus
• Depositing Lab: Catherine Berlot
• Publication: Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.

Backbone

• Vector backbone: Derived from pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3954
• Total vector size (bp): 5925
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Beta-2-adrenergic receptor-mRFP
• Alt name: ADRB2
• Species: H. sapiens (human); Discosoma sp.
• Insert Size (bp): 1971
• Entrez Gene: ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Beta-2-adrenergic receptor-GFP was obtained from Gerda Breitweiser, Weis Center for Research, Geisinger Clinic, Danville, Pa. mRFP was obtained from Roger Tsien, University of California, San Diego, California.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry