pCellFree_G01
(Plasmid
#67135)
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Purpose(Empty Backbone) Gateway destination vector for cell-free expression with N-term 8xHis tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLTE EGFP
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Vector typeCell-free expression
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
8xHis-attR1-ccdB-CamR-attR2-Stop
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCellFree_G01 was a gift from Kirill Alexandrov (Addgene plasmid # 67135 ; http://n2t.net/addgene:67135 ; RRID:Addgene_67135) -
For your References section:
Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems. Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera D, Gonda TJ, Alexandrov K. J Biotechnol. 2015 Feb 10;195:1-7. doi: 10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19. 10.1016/j.jbiotec.2014.12.006 PubMed 25529348
