pCellFree_G03 ZFYVE9
(Plasmid
#67134)
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PurposeStandard for cell-free expression benchmarking.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67134 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCellFree_G03
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Vector typeCell-free expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZFYVE9
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Alt nameZinc finger FYVE domain-containing protein 9
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SpeciesH. sapiens (human)
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MutationF1359I
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Entrez GeneZFYVE9 (a.k.a. MADHIP, NSP, PPP1R173, SARA, SMADIP)
- Promoter T7
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCellFree_G03 ZFYVE9 was a gift from Kirill Alexandrov (Addgene plasmid # 67134 ; http://n2t.net/addgene:67134 ; RRID:Addgene_67134) -
For your References section:
Performance benchmarking of four cell-free protein expression systems. Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K. Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. 10.1002/bit.25814 PubMed 26301602
Included in kit:
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