pCellFree_G03 NFKB1
(Plasmid #67126)

• Purpose: Standard for cell-free expression benchmarking.
• Depositing Lab: Kirill Alexandrov
• Publication: Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.

Backbone

• Vector backbone: pCellFree_G03
• Vector type: Cell-free expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NFKB1
• Alt name: Nuclear factor of kappa light polypeptide gene enhancer
• Species: H. sapiens (human)
• Entrez Gene: NFKB1 (a.k.a. CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB)
• Promoter: T7
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry