pCellFree_G03 PSMC1
(Plasmid #67087)

• Purpose: Standard for cell-free expression benchmarking.
• Depositing Lab: Kirill Alexandrov
• Publication: Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.

Backbone

• Vector backbone: pCellFree_G03
• Vector type: Cell-free expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PSMC1
• Alt name: 26S protease regulatory subunit 4
• Species: H. sapiens (human)
• Mutation: E275G, I287V and R294G
• Entrez Gene: PSMC1 (a.k.a. NEDGTH, P26S4, RPT2, S4, p56)
• Promoter: T7
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry