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Addgene

pCellFree_G03 TFF1
(Plasmid #67047)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCellFree_G03
  • Vector type
    Cell-free expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TFF1
  • Alt name
    Trefoil factor 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    TFF1 (a.k.a. BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • EGFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCellFree_G03 TFF1 was a gift from Kirill Alexandrov (Addgene plasmid # 67047 ; http://n2t.net/addgene:67047 ; RRID:Addgene_67047)

  • For your References section:

    Performance benchmarking of four cell-free protein expression systems. Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K. Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. 10.1002/bit.25814 PubMed 26301602
Included in kit:
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