HaloTag-G protein gamma-1
(Plasmid
#67038)
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PurposeG protein gamma-1 tagged with HaloTag on amino terminus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHT2
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3988
- Total vector size (bp) 5179
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaloTag-G protein gamma-1
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Alt nameGNGT1
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SpeciesB. taurus (bovine)
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Insert Size (bp)1191
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Entrez GeneGNGT1
- Promoter CMV
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Tag
/ Fusion Protein
- HaloTag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGamma-1 cDNA obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville PA. HaloTag pHT2 was purchased from Promega.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gamma-1 insert can be retrieved from pHT2 using Ngo MIV.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HaloTag-G protein gamma-1 was a gift from Catherine Berlot (Addgene plasmid # 67038 ; http://n2t.net/addgene:67038 ; RRID:Addgene_67038)