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Addgene

HaloTag-G protein gamma-1
(Plasmid #67038)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHT2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3988
  • Total vector size (bp) 5179
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloTag-G protein gamma-1
  • Alt name
    GNGT1
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1191
  • Entrez Gene
    GNGT1
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer EBV Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gamma-1 cDNA obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville PA. HaloTag pHT2 was purchased from Promega.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gamma-1 insert can be retrieved from pHT2 using Ngo MIV.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HaloTag-G protein gamma-1 was a gift from Catherine Berlot (Addgene plasmid # 67038 ; http://n2t.net/addgene:67038 ; RRID:Addgene_67038)
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