pRJPaph-mChe
(Plasmid #67034)

• Purpose: Plasmid for Paph-mCherry integration downstream of B. diazoefficiens (B. japonicum) scoI
• Depositing Lab: Julia Vorholt
• Publication: Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Backbone

• Vector backbone: pSUP202pol4
• Vector type: Conjugative bacterial vector

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
• Species: Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)
• Insert Size (bp): 807

Gene/Insert 2

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 816

Resource Information

• Supplemental Documents:
  • GenBank file
• A portion of this plasmid was derived from a plasmid made by: pMRE-105-mCherry (Schada von Borzyskowski et al., ACS, Synth. Biol., 4:430-443, 2015)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry