HaloTag-G protein beta-2
(Plasmid #67017)

• Purpose: G protein beta-2 tagged with HaloTag on amino terminus
• Depositing Lab: Catherine Berlot
• Publication: Berlot Lab HaloTag Fusions (unpublished)

Backbone

• Vector backbone: pHT2
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3988
• Total vector size (bp): 5977
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NM_005273.3
• Species: H. sapiens (human)
• Insert Size (bp): 1989
• GenBank ID: HaloTag-G protein beta-2
• Entrez Gene: GNB2 (a.k.a. HG2C1, SSS4, SSS4; NEDHYDF)
• Promoter: CMV
• Tag / Fusion Protein:
  • HaloTag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EBV Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Beta-2 cDNA obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville PA. HaloTag pHT2 was purchased from Promega.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Beta-2 insert can be retrieved from pHT2 using Ngo MIV.