HaloTag-beta2-adrenergic receptor
(Plasmid #66994)

• Purpose: Beta-2-adrenergic receptor tagged with HaloTag on amino terminus
• Depositing Lab: Catherine Berlot
• Publication: Berlot Lab HaloTag Fusions (unpublished)

Backbone

• Vector backbone: Derived from pHT2
• Backbone size w/o insert (bp): 3989
• Total vector size (bp): 6286
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HaloTag-beta2-adrenergic receptor
• Alt name: ADRB2
• Species: H. sapiens (human)
• Insert Size (bp): 2297
• Mutation: The signal sequence from human growth hormone was added to the N-terminus of HaloTag, which was then added to the amino terminus of the beta2-adrenergic receptor.
• Entrez Gene: ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
• Promoter: CMV
• Tag / Fusion Protein:
  • Human Growth hormone Signal Sequence-HaloTag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EBV Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Beta-2-adrenergic receptor-GFP was obtained from Gerda Breitweiser, Weis Center for Research, Geisinger Clinic, Danville, PA. HaloTag pHT2 was purchased from Promega.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Beta2-adrenergic receptor insert (without the signal sequence) can be retrieved using Ngo MIV.