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pGEX-4T1 Erg (1-208)
(Plasmid #66990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4969
  • Total vector size (bp) 5596
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Erg (1-208)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    627
  • Mutation
    Amino acids from 1-208
  • GenBank ID
    NM_133659.3
  • Entrez Gene
    Erg (a.k.a. D030036I24Rik)
  • Promoter TAC
  • Tag / Fusion Protein
    • GST fusion (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T1 Erg (1-208) was a gift from Christopher Vakoc (Addgene plasmid # 66990 ; http://n2t.net/addgene:66990 ; RRID:Addgene_66990)

  • For your References section:

    BET Bromodomain Inhibition Suppresses the Function of Hematopoietic Transcription Factors in Acute Myeloid Leukemia. Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR. Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 10.1016/j.molcel.2015.04.011. 10.1016/j.molcel.2015.04.011 PubMed 25982114
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