MSCV PIG Erg
(Plasmid
#66984)
-
PurposeRetroviral expression plasmid for mouse Erg expression; containing bicistronic IRES-GFP
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneMSCV PIG
-
Backbone manufacturerAddgene Plasmid #18751
- Backbone size w/o insert (bp) 6300
- Total vector size (bp) 7761
-
Vector typeRetroviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameErg
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1461
-
GenBank IDNM_133659.3
-
Entrez GeneErg (a.k.a. D030036I24Rik)
- Promoter LTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MSCV PIG Erg was a gift from Christopher Vakoc (Addgene plasmid # 66984 ; http://n2t.net/addgene:66984 ; RRID:Addgene_66984) -
For your References section:
BET Bromodomain Inhibition Suppresses the Function of Hematopoietic Transcription Factors in Acute Myeloid Leukemia. Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR. Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 10.1016/j.molcel.2015.04.011. 10.1016/j.molcel.2015.04.011 PubMed 25982114
