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Addgene

MSCV PIG Pu.1
(Plasmid #66982)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV PIG
  • Backbone manufacturer
    Addgene Plasmid #18751
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 7119
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spi1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    819
  • Entrez Gene
    Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer MSCV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV PIG Pu.1 was a gift from Christopher Vakoc (Addgene plasmid # 66982 ; http://n2t.net/addgene:66982 ; RRID:Addgene_66982)

  • For your References section:

    BET Bromodomain Inhibition Suppresses the Function of Hematopoietic Transcription Factors in Acute Myeloid Leukemia. Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR. Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 10.1016/j.molcel.2015.04.011. 10.1016/j.molcel.2015.04.011 PubMed 25982114
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