pcDNA3 Flag Pu.1
(Plasmid #66974)

• Purpose: Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein
• Depositing Lab: Christopher Vakoc
• Publication: Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 10.1016/j.molcel.2015.04.011.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6219
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Spi1
• Species: M. musculus (mouse)
• Insert Size (bp): 819
• Entrez Gene: Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry