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Addgene

pMZ036
(Plasmid #66973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    Mammalian Expression ; zebrafish mRNA injection

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zebrafish Chk1
  • Species
    D. rerio (zebrafish)
  • Mutation
    Q341H mutation compared to GenBank reference sequence NP_956487.1
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMZ036 was a gift from Michael Lampson (Addgene plasmid # 66973 ; http://n2t.net/addgene:66973 ; RRID:Addgene_66973)

  • For your References section:

    Regulation of zygotic genome activation and DNA damage checkpoint acquisition at the mid-blastula transition. Zhang M, Kothari P, Mullins M, Lampson MA. Cell Cycle. 2014;13(24):3828-38. doi: 10.4161/15384101.2014.967066. 10.4161/15384101.2014.967066 PubMed 25558827
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