pMZ030
(Plasmid
#66970)
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PurposeExpresses wild type zebrafish Chk1 with C-term GFP fluorescent tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namewildtype zebrafish Chk1
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SpeciesD. rerio (zebrafish)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byCloned from wildtype zebrafish Chk1 cDNA purchased from ATCC
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMZ030 was a gift from Michael Lampson (Addgene plasmid # 66970 ; http://n2t.net/addgene:66970 ; RRID:Addgene_66970) -
For your References section:
Regulation of zygotic genome activation and DNA damage checkpoint acquisition at the mid-blastula transition. Zhang M, Kothari P, Mullins M, Lampson MA. Cell Cycle. 2014;13(24):3828-38. doi: 10.4161/15384101.2014.967066. 10.4161/15384101.2014.967066 PubMed 25558827
