Beta-2-adrenergic receptor-mCerulean
(Plasmid #66957)

• Purpose: Beta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus
• Depositing Lab: Catherine Berlot
• Publication: Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.

Backbone

• Vector backbone: Derived from pECFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3954
• Total vector size (bp): 5967
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Beta-2-adrenergic receptor-mCerulean
• Alt name: ADRB2
• Species: H. sapiens (human); Aequorea victoria
• Insert Size (bp): 2013
• Entrez Gene: ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCerulean (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Beta-2-adrenergic receptor-GFP was obtained from Gerda Breitweiser, Weis Center for Research, Geisinger Clinic, Danville, PA. Monomeric Cerulean was obtained from David Piston, Vanderbilt University, Nashville, TN.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference for monomeric Cerulean:

Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.