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Addgene

MSCV-FLAG-mMdm2-IRES-GFP
(Plasmid #66955)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Backbone manufacturer
    Addgene 66956
  • Backbone size w/o insert (bp) 6564
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mdm2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    489
  • Entrez Gene
    Mdm2 (a.k.a. 1700007J15Rik, Mdm-2)
  • Promoter MSCV
  • Tags / Fusion Proteins
    • IRES-GFP (C terminal on backbone)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer pCDH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-FLAG-mMdm2-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 66955 ; http://n2t.net/addgene:66955 ; RRID:Addgene_66955)
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