pBSR (mCherry-surrogateTarget-bsr)
(Plasmid #66950)

• Purpose: Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
• Depositing Lab: Silvestro Conticello
• Publication: Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.

Backbone

• Vector backbone: pBS SK
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 6479
• Vector type: Mammalian Expression, Mouse Targeting, CRISPR, TALEN

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: blasticidin
• Insert Size (bp): 1300
• GenBank ID:
• Promoter: beta-actin

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pBS SK βactin mCherryROSAbsr

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry