pErbB2-ECFP
(Plasmid
#66945)
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PurposeMammalian expression of rat ErbB2 tagged with ECFP
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepECFP-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameErbB2
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Alt nameHER2
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SpeciesR. norvegicus (rat)
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Entrez GeneErbb2 (a.k.a. HER2/neu, neu)
- Promoter CMV
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Tag
/ Fusion Protein
- ECFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer EBV reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pErbB2-ECFP was a gift from Mads Lerdrup & Bo van_Deurs (Addgene plasmid # 66945 ; http://n2t.net/addgene:66945 ; RRID:Addgene_66945) -
For your References section:
Geldanamycin stimulates internalization of ErbB2 in a proteasome-dependent way. Lerdrup M, Hommelgaard AM, Grandal M, van Deurs B. J Cell Sci. 2006 Jan 1;119(Pt 1):85-95. Epub 2005 Dec 13. 10.1242/jcs.02707 PubMed 16352662