pUAST-Myc-mSesn2 (1186)
(Plasmid
#66920)
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Purposeexpression of mouse Sesn2 in drosophila
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUAST
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSesn2
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SpeciesM. musculus (mouse)
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Entrez GeneSesn2 (a.k.a. HI95, SEST2, Ses2)
- Promoter HS promoter
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCasper-hs
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAST-Myc-mSesn2 (1186) was a gift from Michael Karin & Jun Hee Lee (Addgene plasmid # 66920 ; http://n2t.net/addgene:66920 ; RRID:Addgene_66920) -
For your References section:
Sestrin as a feedback inhibitor of TOR that prevents age-related pathologies. Lee JH, Budanov AV, Park EJ, Birse R, Kim TE, Perkins GA, Ocorr K, Ellisman MH, Bodmer R, Bier E, Karin M. Science. 2010 Mar 5;327(5970):1223-8. doi: 10.1126/science.1182228. 10.1126/science.1182228 PubMed 20203043