pGEX-4T-1 dSesn (1187)
(Plasmid
#66919)
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Purposebacterial expression of dSesn
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSesn
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SpeciesD. melanogaster (fly)
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Entrez GeneSesn (a.k.a. Dmel_CG11299, BEST:GH20113, BcDNA:GH20113, CG11299, Dmel\CG11299, dSesn)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1 dSesn (1187) was a gift from Michael Karin & Jun Hee Lee (Addgene plasmid # 66919 ; http://n2t.net/addgene:66919 ; RRID:Addgene_66919) -
For your References section:
Sestrin as a feedback inhibitor of TOR that prevents age-related pathologies. Lee JH, Budanov AV, Park EJ, Birse R, Kim TE, Perkins GA, Ocorr K, Ellisman MH, Bodmer R, Bier E, Karin M. Science. 2010 Mar 5;327(5970):1223-8. doi: 10.1126/science.1182228. 10.1126/science.1182228 PubMed 20203043
