N1-LOC8R
(Plasmid #66896)

• Purpose: Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
• Depositing Lab: Georg Ramm
• Publication: Mitochondrial LOC8R Plasmids (unpublished)

Backbone

• Vector backbone: pMK-RQ (kanR)
• Backbone manufacturer: GeneArt (LifeTechnologies)
• Backbone size w/o insert (bp): 2278
• Total vector size (bp): 2679

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LOC8R
• Alt name: Leader Of Cox8 Repeated
• Alt name: Synthetic variants of COX8A MTS
• Species: Synthetic
• Insert Size (bp): 401

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SfiI (not destroyed)
• 3′ cloning site: SfiI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The LOC8R targeting sequence was designed by Benjamin S. Padman during his doctoral research project (Primary supervisor: Georg Ramm)

Insert into an N1 vector by subcloning. The number of Cox8 MTS repeats in LOC8R can be modified as follows:
4x Cox8: Insert at HindIII & AgeI sites. (subclone directly).
3x Cox8: Digest with BamHI and re-ligate. insert at HindIII & AgeI sites.
2x Cox8: Digest with XhoI and re-ligate. insert at HindIII & AgeI sites.
1x Cox8: Digest with HindIII and BamHI. Insert at HindIII & BamHI sites.