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pTER E2F1shRNA human
(Plasmid #66883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTER
  • Backbone manufacturer
    A gift from Dr. Marc van de Wetering (PMID: 12776180)
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2F transcription factor 1
  • gRNA/shRNA sequence
    TATCTGTACTACGCAGCTG
  • Species
    H. sapiens (human)
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
  • Promoter H1 Tet

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pQE promoter
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTER E2F1shRNA human was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 66883 ; http://n2t.net/addgene:66883 ; RRID:Addgene_66883)

  • For your References section:

    Nuclear localization of gamma-tubulin affects E2F transcriptional activity and S-phase progression. Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M. FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25. 10.1096/fj.11-187484 PubMed 21788450
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