pBNJ28.2
(Plasmid #66874)

• Purpose: For transposon tagging with YFP
• Depositing Lab: Thomas Hughes
• Publication: Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Backbone

• Vector backbone: pBlunt-topo
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 3000
• Vector type: source for a Tn7 transposon

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eYFP in a transposon
• Insert Size (bp): 2000
• Tag / Fusion Protein:
  • eYFP

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • step by step protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry