pBNJ24.6
(Plasmid
#66873)
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Purposefor transposon tagging with a fluorescent protein
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66873 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlunt-topo
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 3000
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Vector typesource for a Tn7 transposon
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP in a transposon
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Insert Size (bp)2000
- Promoter none
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Tag
/ Fusion Protein
- eGFP
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBNJ24.6 was a gift from Thomas Hughes (Addgene plasmid # 66873 ; http://n2t.net/addgene:66873 ; RRID:Addgene_66873) -
For your References section:
A faster way to make GFP-based biosensors: two new transposons for creating multicolored libraries of fluorescent fusion proteins. Sheridan DL, Hughes TE. BMC Biotechnol. 2004 Aug 18;4:17. 10.1186/1472-6750-4-17 PubMed 15317651