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Addgene

pBS-IT5
(Plasmid #66869)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK+
  • Total vector size (bp) 3229

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Inntag 5
  • Alt name
    PDB_ID 1WHP
  • Species
    Phleum pratense
  • Insert Size (bp)
    288

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GTTGTG TGGAATTGTGAGCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-IT5 was a gift from Marti Aldea (Addgene plasmid # 66869 ; http://n2t.net/addgene:66869 ; RRID:Addgene_66869)

  • For your References section:

    Inntags: small self-structured epitopes for innocuous protein tagging. Georgieva MV, Yahya G, Codo L, Ortiz R, Teixido L, Claros J, Jara R, Jara M, Iborra A, Gelpi JL, Gallego C, Orozco M, Aldea M. Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556. 10.1038/nmeth.3556 PubMed 26322837
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