pcDNA3.1-StrepHA-SUMO2(Q87R)
(Plasmid #66868)

• Purpose: Mammalian expression of SUMO2(Q87R) with a strep and an HA tag
• Depositing Lab: Ying Liu
• Publication: Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99. doi: 10.1021/pr500997p. Epub 2015 Mar 19.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 5823
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO2
• Alt name: small ubiquitin-like modifier 2
• Species: H. sapiens (human)
• Insert Size (bp): 450
• Mutation: Q87R
• GenBank ID: AAH08450.1
• Entrez Gene: SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Strep (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV For
• 3′ sequencing primer: BGH Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry