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pETM6-M.accABCD
(Plasmid #66865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM6
  • Backbone size w/o insert (bp) 5149
  • Total vector size (bp) 9679
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    accA
  • Species
    E. coli
  • Insert Size (bp)
    960
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGAGTCTGAATTTCCTTG
  • 3′ sequencing primer TTACGCGTAACCGTAGCTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    accB
  • Species
    E. coli
  • Insert Size (bp)
    471
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGGATATTCGTAAG
  • 3′ sequencing primer TTACTCGATGACGACC
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    accC
  • Species
    E. coli
  • Insert Size (bp)
    1350
  • Promoter T7

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGCTGGATAAAATTG
  • 3′ sequencing primer TTATTTTTCCTGAAGACC
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    accD
  • Species
    E. coli
  • Insert Size (bp)
    915
  • Promoter T7

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGAGCTGGATTGAACG
  • 3′ sequencing primer TCAGGCCTCAGGTTCCTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM6-M.accABCD was a gift from Mattheos Koffas (Addgene plasmid # 66865 ; http://n2t.net/addgene:66865 ; RRID:Addgene_66865)

  • For your References section:

    Modular optimization of multi-gene pathways for fatty acids production in E. coli. Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. 10.1038/ncomms2425 PubMed 23361000
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