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Addgene

pPD95.75 RSF-1
(Plasmid #66863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PD95.75
  • Backbone manufacturer
    Addgene
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RSF-1
  • Species
    C. elegans (nematode)
  • Entrez Gene
    rsf-1 (a.k.a. CELE_T24F1.3)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer GFP-R primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

I performed PCR on C.elegans genome to isolate the genome of RSF-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPD95.75 RSF-1 was a gift from Yutaka Hata (Addgene plasmid # 66863 ; http://n2t.net/addgene:66863 ; RRID:Addgene_66863)

  • For your References section:

    Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y. Exp Cell Res. 2013 Feb 1;319(3):1-11. doi: 10.1016/j.yexcr.2012.10.008. Epub 2012 Oct 26. 10.1016/j.yexcr.2012.10.008 PubMed 23103556
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