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Addgene

pClneoFH-let60 G12V
(Plasmid #66860)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pClneo
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Let-60
  • Species
    C. elegans (nematode)
  • Mutation
    Glycine 12 is mutated to valine
  • Entrez Gene
    let-60 (a.k.a. CELE_ZK792.6)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG-His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer CMV promoter
  • 3′ sequencing primer T3 promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoFH-let60 G12V was a gift from Yutaka Hata (Addgene plasmid # 66860 ; http://n2t.net/addgene:66860 ; RRID:Addgene_66860)

  • For your References section:

    Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y. Exp Cell Res. 2013 Feb 1;319(3):1-11. doi: 10.1016/j.yexcr.2012.10.008. Epub 2012 Oct 26. 10.1016/j.yexcr.2012.10.008 PubMed 23103556
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