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Addgene

pClneoMyc' CST-1
(Plasmid #66859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pClneo
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CST-1
  • Species
    C. elegans (nematode)
  • Mutation
    S48N, H50R, S257P and V340A mutations in CST-1 compared to GenBank reference sequence NP_001024575.2. CST-1 ends at amino acid 444
  • Entrez Gene
    cst-1 (a.k.a. CELE_F14H12.4)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing results find amino acid changes S48N and H50R compared to available CST-1 reference sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoMyc' CST-1 was a gift from Yutaka Hata (Addgene plasmid # 66859 ; http://n2t.net/addgene:66859 ; RRID:Addgene_66859)

  • For your References section:

    Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y. Exp Cell Res. 2013 Feb 1;319(3):1-11. doi: 10.1016/j.yexcr.2012.10.008. Epub 2012 Oct 26. 10.1016/j.yexcr.2012.10.008 PubMed 23103556
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