pPD95.75-cYAP-1 S104A
(Plasmid #66858)

• Purpose: Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine
• Depositing Lab: Yutaka Hata
• Publication: Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45. doi: 10.1016/j.yexcr.2013.01.020. Epub 2013 Feb 7.

Backbone

• Vector backbone: PD95.75
• Backbone manufacturer: Addgene
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YAP-1
• Species: C. elegans (nematode)
• Mutation: Serine104 is mutated to alanine
• Entrez Gene: yap-1 (a.k.a. CELE_F13E6.4)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: GFP-R primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine.