pClneoMyc'-cYAP-1
(Plasmid
#66854)
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PurposeExpress Myc-tagged C.elegans YAP-1 in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepClneo
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Backbone manufacturerPromega
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYAP-1
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SpeciesC. elegans (nematode)
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Entrez Geneyap-1 (a.k.a. CELE_F13E6.4)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer CMV promoter
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene sequencing results find amino acid changes Q48R and Y213F compared to available YAP-1 reference sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pClneoMyc'-cYAP-1 was a gift from Yutaka Hata (Addgene plasmid # 66854 ; http://n2t.net/addgene:66854 ; RRID:Addgene_66854) -
For your References section:
Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans. Iwasa H, Maimaiti S, Kuroyanagi H, Kawano S, Inami K, Timalsina S, Ikeda M, Nakagawa K, Hata Y. Exp Cell Res. 2013 Apr 15;319(7):931-45. doi: 10.1016/j.yexcr.2013.01.020. Epub 2013 Feb 7. 10.1016/j.yexcr.2013.01.020 PubMed 23396260